ROR1-targeted delivery of miR-29b results in epigenetic reprogramming with p21-dependent cell cycle arrest

RNAseq analysis of purified splenic CD19+ B lymphocytes from the hRORxTCL1 mouse model treated with 2A2-miR-29b-ILP or 2A2-scramble-ILP. Moreover, 128 of the 233 differentially expressed genes are related to cell growth and proliferation. Gene expression profiling of engrafted murine leukemia identified reprogramming of cell cycle regulators with decreased SP1 and increased p21 expression following miR-29b treatment. The experiment was further confirmed by protein modulation leading to cell cycle arrest.