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Purkinje neuron injected with Lucifer Yellow.
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2013-03-08
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Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons
The use of genetically encoded ‘self-labeling tags’ with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesi
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Puncta surrounding pyramidal neurons somata.
(a)Puncta density data are expressed as mean ± s.e.m. of puncta expressing the named marker/s per micron of soma perimeter. (b)Co-localization percentage of puncta expressing the first named marker wh
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Quantification of GFP labeled neurons.
Cross sections of HH24 electroporated embryos were stained with cell fate markers. Interneurons (IN) were stained with Lhx2/9, Lhx1, Isl1, Brn3a and motor neurons (MN) with Isl1. Progenitor cells that
Figshare2015-12-02 更新20
Additional file 4 of Integrated single cell data analysis reveals cell specific networks and novel coactivation markers
Table S1. This xlsx file contains candidate mature markers with unknown olfactory/neuronal expression and/or function. (XLSX 8.15 KB)
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ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
The correct subcellular distribution of proteins establishes the complex morphology and function of neurons. Fluorescence microscopy techniques are invaluable to investigate subcellular protein distri
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