Supplementary material.docx

Experimental.Plant materials and phytopharmaceutical productChemicals and apparatusPreparation of Standards and samplesInstrumentation and Chromatographic conditionsFiguresOptimisation of chromatographic conditionsS1:Effect of organic solvent type in the mobile phase on separation S2:Effect of initial and final concentration of ACN in the mobile phase on separation S3:Effect of gradient time on separation S4:Effect of column temperature on separation S5:Effect of flow rate on corrected peak area S6:Peak characterization in the crude extracts Indirect-dispersive liquid-liquid microextraction S7: Effect of IDLLME on the baseline, (a) before (b) after IDLLME, Peaks: 1, vitexin 2''-O-rhamnoside, 2, rutin, 3, vitexin, and 4, hyperoside. TablesS1:Optimum chromatographic conditions Determination of analytes in real samples S2:Concentration of analytes in the real samples

实验部分。
植物材料与植物源药剂。
化学品与仪器设备。
标准品与样品制备。
仪器分析与色谱条件。
附图。
色谱条件优化。
S1：流动相有机溶剂种类对分离效果的影响
S2：流动相初始与终浓度乙腈（ACN）对分离效果的影响
S3：梯度洗脱时间对分离效果的影响
S4：柱温对分离效果的影响
S5：流速对校正峰面积的影响
S6：粗提物中的峰表征
S7：间接分散液液微萃取（Indirect-dispersive liquid-liquid microextraction, IDLLME）对基线的影响：(a) 萃取前，(b) 萃取后；色谱峰：1. 牡荆素2''-O-鼠李糖苷，2. 芦丁，3. 牡荆素，4. 金丝桃苷。
表S1：最优色谱条件
实际样品中分析物的测定
S2：实际样品中分析物的浓度