GNPS - New actinomycin produced by Streptomyces sp. MBT27

In the current study Streptomyces sp. MBT27, strain isolated from remote area of Qinling mountains, was fermented with different carbon sources and metabolomic analysis of secondary metabolites was carried out by MS and multivariate data analysis. The statistical analysis suggested that extracts with stronger antimicrobial activity against B. subtilis contained higher concentrations of actinomycins. Further analyses of the metabolic profiles by oPLS-DA and GNPS molecular networking resulted in the isolation of a novel actinomycin analog, actinomycin L1 and L2 and three known actinomycins D, X0beta and X2.