Synchronous fluorescence as a green and selective tool for simultaneous determination of bambuterol and its main degradation product, terbutaline

A green, sensitive and cost-effective method is introduced in this research for the determination of bambuterol and its main degradation product; terbutaline simultaneously relied on the synchronous spectrofluorimetric technique. First derivative synchronous spectrofluorimetric amplitude is measured at Δλ=20 nm, so bambuterol can be quantitated at 260 nm, and terbutaline can be measured at 290 nm, each at the zero crossing point of the other. The amplitude-concentration plots were linear over the concentration ranges of 0.2-6.0 g/mL and 0.2-4.0 g/mL for both bambuterol and terbutaline, respectively. Official guidelines were followed to calculate the validation parameters of the proposed method. The low values of LODs 0.023, 0.056 g/mL and LOQs 0.071, 0.169 g/mL for bambuterol and terbutaline, respectively point out to the sensitivity of the method. Bambuterol is a prodrug for terbutaline, and the latter is considered its degradation product so the established method could be regarde...

本研究提出了一种绿色、灵敏且经济高效的分析方法，可同步测定班布特罗（bambuterol）及其主要降解产物特布他林（terbutaline），该方法基于同步荧光光谱技术。在Δλ=20 nm条件下测定一阶导数同步荧光强度，班布特罗可在260 nm处实现定量，特布他林则可在290 nm处定量，二者均处于对方的零交点位置。班布特罗与特布他林的强度-浓度校准曲线分别在0.2~6.0 μg/mL和0.2~4.0 μg/mL的浓度范围内呈良好线性关系。本方法严格遵循官方指南计算所提方法的验证参数，班布特罗与特布他林的检出限（Limit of Detection）分别为0.023 μg/mL和0.056 μg/mL，定量限（Limit of Quantitation）分别为0.071 μg/mL和0.169 μg/mL，数值均较低，表明该方法具备优异的灵敏度。班布特罗是特布他林的前体药物，而特布他林被视为班布特罗的降解产物，因此本研究建立的方法可用于……