Whole-genome resequencing of BC1 populations from Arabidopsis (Col x Ler) x Col crosses (scep1)

This experiment aims at analyzing crossover distribution in female and male meiosis, in the Arabidopsis scep1 compared to wild type. The heterozygous scep1-1+/- plants were crossed with heterozygous scep1-7+/- plants to produce scep1-1/scep1-7 heteroallelic homozygous mutant. These plants were backcrossed as male or female with wild-type Col to generate the sequencing populations. Leaf samples from the back-crossed population were used for DNA purification and library preparation for Illumina sequencing (HiSeq 3000 2 × 150 base pairs [bp]), performed at the Max Planck-Genome-center (https://mpgc.mpipz.mpg.de/home/).