Interleukin-6 from the Adipose Secretome Potentiate Differentiation of Adipose Progenitors Through Activation of Redox Signaling

<b>Supplementary Figure 1. Adipogenic Stimulus Increases the Number of Short Mitochondria.</b> (A) Box plot of mitochondrial length analyzed by category, short, intermediate, or elongated for APCs treated adipogenic stimulus or vehicle normalized to cell number per slide in mitotracker-stained APCs (B) Representative images of mitotracker stained APCs.<b>Supplementary Figure 2. Conditioned Media Treatment Increases Adipogenesis.</b> (A) Eluted Oil Red O absorbance for cells treated with CD CM or HFD CM from male subcutaneous adipose tissue explants or vehicle on day 7 of differentiation (n=4). * p ≤ 0.05.<b>Supplementary Figure 3. IL-6 and TNF Identified as Upstream Regulators </b>(A) Network of IL-6 as an upstream regulator of differentially expressed proteins found in our dataset in IPA. (B) Network of TNFa as an upstream regulator of differentially expressed proteins found in our dataset in IPA.<b>Supplementary Figure 4. IL-6 Treatment Increases Adipogenesis.</b> (A) Eluted Oil Red O absorbance from APCs treated with IL-6 [0.1, 1, 5 ng/mL] (n=4). * p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.0001.<br>

<b>补充图1. 成脂诱导刺激可增加短线粒体的数量</b> (A) 针对经成脂诱导刺激或溶剂对照处理的脂肪祖细胞（Adipocyte Progenitor Cells, APCs），按线粒体长度分为短、中等、细长三类进行统计的箱线图，样本经Mitotracker探针染色，结果已按每张玻片的细胞数进行归一化。(B) 经Mitotracker探针染色的脂肪祖细胞代表性成像图。<b>补充图2. 条件培养基处理可促进成脂分化</b> (A) 于分化第7天，对经雄性皮下脂肪组织外植体的对照饮食条件培养基（CD CM）、高脂饮食条件培养基（HFD CM）或溶剂对照处理的细胞，检测其洗脱后的油红O吸光度（n=4）。*p ≤ 0.05。<b>补充图3. IL-6与TNF被鉴定为上游调控因子</b> (A) 在Ingenuity通路分析（IPA）中，IL-6作为本数据集所获差异表达蛋白的上游调控因子所构建的调控网络。(B) 在Ingenuity通路分析（IPA）中，TNFa作为本数据集所获差异表达蛋白的上游调控因子所构建的调控网络。<b>补充图4. IL-6处理可促进成脂分化</b> (A) 经浓度为0.1、1、5 ng/mL的IL-6处理的脂肪祖细胞，其洗脱后的油红O吸光度检测结果（n=4）。*p ≤ 0.05，**p ≤ 0.01，***p ≤ 0.001，****p ≤ 0.0001。<br>