Strain‐dependent differences in coordination of yeast signalling networks

The yeast mitogen activated protein kinase pathways serve as a model system for understanding how network interactions affect the way in which cells coordinate the response to multiple signals. We have quantitatively compared two yeast strain backgrounds YPH499 and Σ1278b (both of which have previously been used to study these pathways) and found several important differences in how they coordinate the interaction between the high osmolarity glycerol (HOG) and mating pathways. In the Σ1278b background, in response to simultaneous stimulus, mating pathway activation is dampened and delayed in a dose dependent manner. In the YPH499 background, only dampening is dose dependent. Further, leakage from the HOG pathway into the mating pathway (crosstalk) occurs during osmostress alone in the Σ1278b background only. The mitogen activated protein kinase Hog1p suppresses crosstalk late in an induction time course in both strains but does not affect the early crosstalk seen in the Σ1278b backgroun..., Data was collected as described in the Materials and Methods section of Scott, et al., AMN1_allele_swap: Representative images of AMN1 wild-type (yMM1174) and amn1 (yMM1584) Sigma S. cerevisiae strains. Presented in Figure 8 of Scott, et al. The .tif files can be opened in ImageJ.
flow_cytometry: Contains the raw Flow Cytometry Standard (.fcs files) for experiments that generated the data for Figures 2D, 3B-F, 4A-B, 5,6,7. FlowJo workspace files (.wsp) as well as summary analysis files (.xlsx) and supplementary files (e.g. plate maps, .xlsx) are also included. The .fcs file can be opened with a variety of flow cytometry software, including FlowJo and open-source options such as FlowPy. The .wsp files require the propriety software FlowJo but are not necessary for analyzing the .fcs files.  The .xlsx files can be opened with Excel, Google Sheets or LibreOffice.
Subfolders are as follows:

crosstalk_time_courses: Data used to generate Figures 5,6,7
double_stimulus_time_courses: Data used to generate Figures 3B-F
sorbitol_dose_response: Data used to generate Figures 2D, 4A-B...

酿酒酵母丝裂原活化蛋白激酶（mitogen activated protein kinase, MAPK）通路是研究网络相互作用如何调控细胞协调多种信号响应的经典模型系统。本研究定量比较了此前均被用于研究此类通路的两种酵母菌株背景YPH499与Σ1278b，发现二者在协调高渗甘油（high osmolarity glycerol, HOG）通路与交配通路的相互作用时存在多处关键差异。

在Σ1278b菌株背景中，当受到双重刺激时，交配通路的激活会以剂量依赖的方式被抑制且延迟；而在YPH499菌株背景中，仅通路抑制作用呈现剂量依赖性。此外，仅在Σ1278b背景的单纯渗透压胁迫条件下，会发生HOG通路向交配通路的渗漏（串扰，crosstalk）。

丝裂原活化蛋白激酶Hog1p可在两种菌株的诱导时间进程后期抑制串扰，但无法影响Σ1278b背景中观察到的早期串扰……

数据采集方式详见Scott等人发表论文的《材料与方法》部分。AMN1_allele_swap：AMN1野生型（yMM1174）与amn1（yMM1584）Σ型酿酒酵母菌株的代表性图像，该内容展示于Scott等人的图8中。.tif格式文件可通过ImageJ打开。

flow_cytometry：包含为生成图2D、3B-F、4A-B、5、6、7的数据实验所对应的原始流式细胞术标准（Flow Cytometry Standard, FCS）文件。同时附带FlowJo工作区文件（.wsp）、汇总分析文件（.xlsx）以及补充文件（如板位图.xlsx等）。.fcs格式文件可通过多种流式细胞术软件打开，包括FlowJo以及FlowPy等开源工具。.wsp格式文件需要专有软件FlowJo才能加载，但并非分析.fcs文件的必需文件。.xlsx格式文件可通过Excel、Google Sheets或LibreOffice打开。

子文件夹结构如下：
crosstalk_time_courses：用于生成图5、6、7的实验数据
double_stimulus_time_courses：用于生成图3B-F的实验数据
sorbitol_dose_response：用于生成图2D、4A-B的实验数据……