Quantitative phytoplankton profiles for Pensacola Transect Station P3 collected during the R/V Bellows cruise of DeSoto Canyon, May 31, 2014

This dataset reports phytoplankton species abundance data for samples collected from the R/V Bellows during the May 31-June 1, 2014 cruise. Samples were taken at Pensacola Transect Station P3 (30.0833 N, 87.25 W) in the Florida Panhandle Bight at the head of DeSoto Canyon. One-liter samples were collected from the surface and a depth 30 meters using Niskin bottles. Up to one liter of each sample was filtered onto a 0.45 micrometer nitrocellulose filter and air-dried. A portion of each filter was mounted on an aluminum stub, sputter-coated, and examined with a JEOL JSM-6480 LV scanning electron microscope. Diatoms, coccolithophores, and armored dinoflagellates were identified to the lowest possible taxonomic category. The number of cells of each taxon in a field of view at a magnification of 1000x was recorded, with up to 700 fields-of-view examined, depending on the density of phytoplankton in the sample. The dataset includes the depth, the volume filtered, the number of cells of each taxon counted, and the calculated abundance of each taxon in cells per liter. Micrographs taken during the analysis are included.

本数据集收录了2014年5月31日至6月1日期间，由R/V贝尔洛斯号（R/V Bellows）研究船航次采集的浮游植物物种丰度数据。采样点位位于德索托峡谷头部的佛罗里达潘汉德尔湾（Florida Panhandle Bight）内的彭萨科拉断面P3站（30.0833°N，87.25°W）。本次研究采用尼斯金采水器（Niskin bottles），分别采集表层及30米水深的1升水样。每份样品取至多1升体积进行过滤，滤膜采用孔径0.45微米的硝酸纤维素膜，过滤完成后经风干处理。取部分滤膜粘贴至铝制样品台，经喷金镀膜后，使用JEOL JSM-6480 LV型扫描电子显微镜进行观测。对硅藻、颗石藻及具壳甲藻（armored dinoflagellates），均鉴定至尽可能低的分类阶元。在1000倍放大倍数下，记录每个视野内各分类单元的细胞数量；依据样品中浮游植物的密度，最多可观测多达700个视野。本数据集包含采样水深、过滤水样体积、各分类单元的计数细胞数，以及计算得到的每升水样中各分类单元的细胞丰度。数据集同时涵盖分析过程中拍摄的显微照片。