Additional file 2 of Gene expression variability in long-term survivors of childhood cancer and cancer-free controls in response to ionizing irradiation

Additional file 2. Results of the gene expression variability classification. a Gene classifications in whole data set (WDS) and classifications after cross-validation (cv) in fibroblasts of cancer-free controls (N0), childhood cancer survivors without (N1) and with at least one second primary neoplasm (N2 +) after exposure 0 Gray. 1 = Hypo-Variable, 2 = Non-Variable, 3 = Hyper-Variable. NA = Gene excluded by bimodal test/NA in CV = classification from WDS not verified, set to 2 for further analyses. b Gene classifications in whole data set (WDS) and classifications after cross-validation (cv) in fibroblasts of cancer-free controls (N0), long-term survivors of childhood cancer without (N1) and with at least one second primary neoplasm (N2 +) after exposure to 0.05 Gray. 1 = Hypo-Variable, 2 = Non-Variable, 3 = Hyper-Variable. NA = Gene excluded by bimodal test/NA in CV = classification from WDS not verified, set to 2 for further analyses. c Gene classifications in whole data set (WDS) and classifications after cross-validation (cv) in fibroblasts of cancer-free controls (N0), long-term survivors of childhood cancer without (N1) and with at least one second primary neoplasm (N2 +) after exposure to 2 Gray. 1 = Hypo-Variable, 2 = Non-Variable, 3 = Hyper-Variable. NA = Gene excluded by bimodal test/NA in CV = classification from WDS not verified, set to 2 for further analyses. d Results of the various employed algorithms for the classification of N1 and N2 + using 30 genes that were only classified as hyper-variable in N2 + post-LDIR. Models were trained and tested using only N1 and N2 + data post-LDIR.

附加文件2：基因表达变异分类结果。
a 全数据集（whole data set, WDS）的基因分类，以及无癌对照（N0）、未发生至少1次第二原发肿瘤的儿童癌症幸存者（N1）与发生至少1次第二原发肿瘤的儿童癌症幸存者（N2+）的成纤维细胞在0戈瑞（Gray）照射后的交叉验证（cross-validation, cv）分类结果。分类定义如下：1=低变异基因（Hypo-Variable），2=非变异基因（Non-Variable），3=高变异基因（Hyper-Variable）。NA=经双峰检验（bimodal test）排除的基因/交叉验证中出现NA=全数据集分类未得到验证，后续分析中将其赋值为2。
b 全数据集（WDS）的基因分类，以及无癌对照（N0）、未发生至少1次第二原发肿瘤的儿童癌症长期幸存者与发生至少1次第二原发肿瘤的儿童癌症长期幸存者（N2+）的成纤维细胞在0.05戈瑞照射后的交叉验证（cv）分类结果。分类定义同a部分。
c 全数据集（WDS）的基因分类，以及无癌对照（N0）、未发生至少1次第二原发肿瘤的儿童癌症长期幸存者与发生至少1次第二原发肿瘤的儿童癌症长期幸存者（N2+）的成纤维细胞在2戈瑞照射后的交叉验证（cv）分类结果。分类定义同a部分。
d 针对仅在低剂量电离辐射（low-dose ionizing radiation, LDIR）照射后N2+组中被归类为高变异基因的30个基因，对N1与N2+组进行分类的多种采用的算法的结果。本分类模型仅使用低剂量电离辐射照射后的N1与N2+组数据进行训练与测试。