Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Fibroblasts_after_irr]

The goal of this study was to understand the role of fibroblasts in thymic regeneration following irradiation-induced acute involution. For this purpose we treated WT mice with sublethal irradiation, sorted fibroblasts from their thymi 3 days after treatment when the thymus begins its recovery, or at an earlier time point (1 day) for comparison, and performed bulk RNA Seq. Young male WT mice were treated with sublethal irradiation 3 days/1 day before sacrifice, and then fibroblasts were isolated and mRNA profiles were generated by deep sequencing using the MARSseq method. 5 replicates were used. The control was untreated age-matched WT mice.