Nicotiana tabacum leaf transcriptome

De novo assembled transcriptome was developed from leaf samples of wild-type (wt) and high oil (HO) parent (plants metabolically engineered to produce oil in leaf tissues). This was used as a reference for differential expression analysis of wt vs HO.

本研究以野生型（wild-type，wt）与高油（high oil，HO）亲本的叶片样本为材料，构建得到从头组装的转录组（de novo assembled transcriptome）；其中高油亲本为经代谢工程改造可在叶片组织中合成油脂的植株。该转录组被用作野生型与高油亲本间差异表达分析的参照序列。