Dataset on fecal microbiota transplantation of type 1 diabetic mice treated with vancomycin

Disturbance of the gut microbiota has been associated with type 1 diabetes (T1D), but the effect of gut dysbiosis on cognitive function in T1D and its potential mechanism remain unknown. In this study, we treated streptozotocin-induced diabetic mice with vancomycin (Vanc) to disturb the gut microbiota and metabolic phenotype. In order to examine the gut microbial changes, we used a 16S rRNA gene sequencing approach to analyze the gut microbiome in caecum contents of normal control (CON), type 1 diabetic (T1D), and vancomycin-treated type 1 diabetic (T1DV) mice at 3, 7 and 11 weeks. To further determine the effect of antibiotic exposure on the gut microbiota at the species level, we performed a metagenomic analysis of the bacteria in caecum contents. Additionally, to study antibiotic effect on host metabolism, a ¹H NMR-based metabolomics approach was applied to analyze metabolic changes in caecum contents, feces, caecum tissues, colonic tissues, serum, and hippocampus. To establish a causal link between the gut microbiota and host metabolism, we next transferred fecal material from healthy donor mice (COND) to T1DV recipients for 14 days.

肠道菌群紊乱与1型糖尿病（type 1 diabetes, T1D）存在关联，但肠道菌群失调对1型糖尿病小鼠认知功能的影响及其潜在机制仍未明确。本研究通过万古霉素（vancomycin, Vanc）干预链脲佐菌素诱导的糖尿病小鼠，以扰乱其肠道菌群与代谢表型。为探究肠道菌群的变化特征，我们分别于3周、7周和11周三个时间点，采用16S rRNA基因测序技术，分析正常对照组（CON）、1型糖尿病模型组（T1D）以及万古霉素干预的1型糖尿病模型组（T1DV）小鼠盲肠内容物中的肠道微生物组。为进一步从物种水平明确抗生素暴露对肠道菌群的影响，我们对盲肠内容物中的细菌进行了宏基因组分析。此外，为研究抗生素对宿主代谢的调控作用，我们采用基于氢谱核磁共振（¹H NMR）的代谢组学方法，对小鼠盲肠内容物、粪便、盲肠组织、结肠组织、血清及海马体的代谢变化进行检测。为建立肠道菌群与宿主代谢间的因果关联，我们随后将健康供体小鼠的粪便样本（COND）移植至T1DV模型小鼠体内，持续干预14天。