Regulation of gene expression in Caco-2 cells after cyclosporine treatment.

Gene expression was measured by RT-PCR in Caco-2 cells 72 h after treatment with 25 µM (30 µg/ml) cyclosporine (n = 3, respectively) and was determined relative to expression of mitATPase6, which served as a housekeeping gene. Gene expression in untreated Caco-2 cells was set to 100 and values for cyclosporine treatment represent transcript abundance relative to control. Non-parametric Mann-Whitney-U-Test was used to compare cyclosporine treated and control groups. Results are considered significant at p