Point coordinate data showing spatial distribution of corticostriatal, corticothalamic, corticocollicular, and corticopontine projections in transgenic mice (v1)

The data set comprises three-dimensional point coordinate data representing the spatial distribution of subcortical projections from 11 locations in the cerebral cortex to the striatum, thalamus, superior colliculus, and pontine nuclei in transgenic mice (Scnn1a-Tg3-Cre, Trib2-F2A-CreERT2, Rbp4-Cre_KL100, Sim1-Cre_KJ18 Tlx3-Cre_PL56). The point data are derived from public high-resolution images of anterogradely labeled axonal projections from different cerebro-cortical locations to four subcortical brain regions. Serial two-photon tomography images and derived segmented images series were programmatically downloaded via API from the Allen Brain Atlas Data Portal. The spatial registrations of the images with the Allen mouse brain atlas Common Coordinate Framework 3 were slightly adjusted to improve anatomical correspondence in regions of interest. The segmented images were binarized, and pixels representing tracer labelling within the caudate-putamen, thalamus, superior colliculus, and pontine nuclei were converted to 3-D point coordinates. The data points are suitable for co-visualization of different combinations of data sets, for comparison the topographic arrangement of visual corticostriatal, corticothalamic, corticocollicular, and corticopontine projections.

本数据集包含三维点坐标数据，用于表征转基因小鼠（品系包括Scnn1a-Tg3-Cre、Trib2-F2A-CreERT2、Rbp4-Cre_KL100、Sim1-Cre_KJ18、Tlx3-Cre_PL56）中大脑皮层11个区域向纹状体、丘脑、上丘及桥核的皮层下投射的空间分布。该点坐标数据源自公开的高分辨率图像，这些图像记录了不同皮层区域向四个皮层下脑区的顺行标记轴突投射。研究团队通过应用程序编程接口（API）从艾伦脑图谱数据门户（Allen Brain Atlas Data Portal）批量下载了连续双光子断层扫描图像及其衍生的分割图像序列。为优化目标感兴趣区的解剖学对应关系，对图像与艾伦小鼠脑图谱通用坐标框架3（Common Coordinate Framework 3）的空间配准结果进行了小幅调整。对分割图像进行二值化处理后，将尾状核-壳核、丘脑、上丘及桥核内示踪剂标记的像素转换为三维点坐标。该数据点集可用于不同数据集组合的协同可视化，也可用于对比视觉皮层的皮层-纹状体、皮层-丘脑、皮层-上丘及皮层-桥核投射的拓扑排布格局。