Types of TGF-β receptor-expressing cells based on double labeling with cell type markers.

Double labeling was performed by in situ hybridization histochemistry for the TGF-β receptors and immunohistochemistry for markers of different cell types at 72 h after MCAO. To identify the cell types the following markers were used: NeuN for neurons, S-100 for astroglial cells, Iba1 for microglial cells, and vWF for endothels. Data on TGF-β receptors and double labeled cells are presented in 3 different locations. The cell counts were performed in 400 µm×400 µm rectangular-shaped areas of the intact (away from the lesion), penumbral and lesioned cerebral cortex from 4 brains.Types of TGF-β receptor-expressing cells based on double labeling with cell type markers.