RXR-Mediated Remodeling of Transcriptional and Chromatin Landscapes in APP Mouse Brain: Insights from Integrated Single-Cell RNA and ATAC Profiling

Ligand activation of nuclear receptor has been linked with therapeutic implications in Alzheimer's Disease. In this study, we used AD mouse model treated with Bexarotene, an RXR agonist and performed scRNA-seq and snATAC-seq using the brain tissue. We reavealed changes in chromatin architure in brain of AD-lie mouse model in response to RXR ligand and link those changes to the initiation of transcriptional programs at a single-cell resolution. Overall design: We used an AD mouse model treated with Bexarotene, an RXR agonist. scRNA-seq and snATAC-seq were performed using mouse brain 10-day post-treatment to assess brain transcriptome and chromatin accessibility change in response to RXR activation. The results were further validated by ChIP-seq.