Additional file 3: of Inhibition of CREB binding protein-beta-catenin signaling down regulates CD133 expression and activates PP2A-PTEN signaling in tumor initiating liver cancer cells

Figure S1. Effect of ICG001 on activation of ERK1/2. Western blot analysis for phospho-ERK and total ERK in DMSO and ICG001 treated samples. Protein levels were normalized to beta-actin and ratio of pERK1/2 to total ERK1/2 was calculated to determine the level of activation. Results represent four independent experiments. Densitometry quantification of protein intensity was performed by Image J Software, NIH. (ZIP 319 kb)