Expression data from reconstructed human epidermis

Gene expression profiles of reconstructed human epidermis following exposure to interleukins 4, 13, and 25 for 48 hours We used microarrays to study the changes in gene expression of reconstructed human epidermis after 48 hours of exposure to interleukins 4, 13, and 25 Reconstructed human epidermal tissue samples at 14 days of culture at the air-liquid interface were treated with Th2 interleukins 4, 13, and 25, during 48 hours. At the end of the experiments, the total RNA of each sample were extracted using the Qiagen RNeasy kit and processed for hybridization on GeneChip Human Gene 2.0 ST arrays (Affymetrix, 902112)