Quantitative PCR determination of spR-12 in somatic and germ line cells.

*Expression relative to that of miR-16.**Expression relative to actual number of cells. Serial dilutions of synthetic spR-12 RNA were used to generate a standard curve of the log of template quantity versus threshold cycle (C(t)). The concentration of RNA ranged from 10–106 initial copies. A minimum of eight oocytes and/or embryos was used in each experiment. Each value corresponds to three or four independent experiments, performed in duplicate. The analysis for spR-13 was not done as it was not possible to synthesize the spR-13 RT-primer.