Gene expression profile at single cell level of lungs of adult male and female C57BL/6 mice with and without high-fat diet-induced obesity

Obesity is associated with various lung pathologies. We performed single nucleus RNA sequencing of formalin-fixed lungs of mice to obtain insights on this. Groups of male and female mice of about 6 months age had been kept on ad libitum for about 3 months on high-fat or control diet (Bioserv products S3282 and F4031, with 60% and 10% fat calories respectively), when they were sacrificed with carbon dioxide-based euthanasia. Lungs were immediately dissected, rinsed in PBS, and the left lower lung lobes flash-frozen in liquid nitrogen. Nuclei isolated from frozen tissues were used for sequencing.肥胖与多种肺部病理状态密切相关。为阐明二者的潜在关联机制，我们对福尔马林固定的小鼠肺部组织开展了单细胞核RNA测序（single nucleus RNA sequencing）。实验选取约6月龄的雌雄小鼠各若干组，分别饲喂高脂饲料与对照饲料（均为Bioserv公司产品，型号分别为S3282、F4031，脂肪供能占比分别为60%与10%），自由采食持续约3个月后，采用二氧化碳法实施安乐死。随后立即解剖取出肺部组织，经磷酸盐缓冲液（PBS）冲洗后，取左侧肺下叶置于液氮中速冻。从冷冻组织中分离得到的细胞核用于本次测序实验。