Additional file 1 of Pediococcus acidilactici reduces tau pathology and ameliorates behavioral deficits in models of neurodegenerative disorders

Additional file 1: Supplemental Fig. 1. (A) Quantification of histological colitis scores in the four groups (8 mice each group).One-way ANOVA with post-hoc LSD test was used for significance. (B) The speed of mice in the Barnes Maze and Y Maze. One-way ANOVA with post-hoc LSD test was used for significance. (C) Western blotting for detecting the protein levels of tau and p-tau in the hippocampus and cortical tissue of the mice (5 mice each group). (D) Exhibition of behavior including pumping rates, head swing rates and body bending rates in PA- or E. coli OP50-treated C.elegans VH254.Paired t-tests were used for significance. (E) Quantification of immunofluorescence intensity for p-tau proteins. Paired t-tests were used for significance. (F) In vitro acid resistance of PA. (G) In vitro bile salt tolerance of PA. Supplemental Fig. 2. Representative IHC sections showing that PA decreased TF, TAU and slc22a17 expression in the brain prefrontal cortex regions of the three groups. Supplemental Fig. 3. Representative IHC sections of TF deposition in brain hippocampal regions of the three groups. Supplemental Table 1. Primers used for qRT-PCR analysis. Supplemental Table 2. Antibodies used in this study. Supplemental Table 3. ELISA kits and biochemical kits used in this study.

附加文件1：补充图1。(A) 四组小鼠（每组8只）的组织学结肠炎评分定量分析，采用单因素方差分析结合事后LSD检验进行显著性检验。(B) 小鼠在巴恩斯迷宫（Barnes Maze）与Y迷宫中的活动速度，采用单因素方差分析结合事后LSD检验进行显著性检验。(C) 采用免疫印迹（Western blotting）检测小鼠海马与皮层组织中tau蛋白及磷酸化tau（p-tau）的蛋白水平，每组包含5只小鼠。(D) 经PA或大肠杆菌OP50处理的秀丽隐杆线虫VH254的行为表现包括蠕动速率、摆头速率与躯体弯曲速率，采用配对t检验进行显著性检验。(E) 磷酸化tau蛋白免疫荧光强度的定量分析，采用配对t检验进行显著性检验。(F) PA的体外耐酸性实验。(G) PA的体外胆盐耐受性实验。
补充图2：代表性免疫组化（IHC）切片结果显示，PA可降低三组小鼠大脑前额叶皮层区域中TF、TAU及slc22a17的表达水平。
补充图3：三组小鼠大脑海马区TF沉积情况的代表性免疫组化切片。
补充表1：qRT-PCR分析所用引物。
补充表2：本研究使用的抗体。
补充表3：本研究使用的ELISA试剂盒与生化试剂盒。