Have you tried spermine?. DSS/spermine
收藏NIAID Data Ecosystem2026-03-10 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJEB17622
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资源简介：
The Dextran Sulfate Sodium (DSS) induced colitis mouse model is commonly used to investigate human inflammatory bowel disease (IBD). Nucleic acid extracts originating from these animals are often contaminated with DSS, which is a strong inhibitor of many enzymatic based molecular biology reactions including PCR and reverse-transcription. Methods for removing DSS from nucleic acids extracts exists for RNA, but no effective protocol for DNA is currently available.We demonstrate a cost effective and easy method to counteract DSS inhibition in RNA extracts and the only existing method, that can be interchangeably applied for DNA samples. The influence of our method on amplicon sequencing of 16S rRNA gene can be retrieved from ENA
创建时间：
2017-03-01
