RIP-seq of SRSF7 in Huh7

RNA seq was performed after IP of Ab-SRSF7 in Huh7 cells. RIP assay was performed on Huh7 cells by LC-Bio (Hangzhou, China). Briefly, Huh7 cells were lysed with cell lysis buffer. The 10% lysis sample was used for input, and 90% was used for immunoprecipitation with anti-SRSF7 antibody (Catalog No. ab137247, Abcam). TRIzol reagent was used for extraction of RNA. The stranded RNA sequencing library was performed by Stranded mRNA Library Prep Kit for Illumina based on the manufacture’s instruction. The duplication bias in PCR and sequencing steps was eliminated by using unique molecular identifier (UMI) of 8 random bases to label the pre-amplified cDNA molecules. The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Illumina Novaseq™ 6000 with PE150 model.