HeV genes F, G, and FG combination transfection transcriptomics profile on HEK29T cells

To delineate cellular processes involved in HeV F, G, and FG combination, we have employed whole genome microarray expression profiling as a discovery platform to identify the upregulated and downregulated genes. Total RNA was collected from transfected cells and untransfected controls. Cy3 labeled cRNA is used for hybridization platform Agilent Human 4 x 44K gene expression array. Time-course based transfections were performed at 6h, 12h, and 21h post-transfection time-points. These transfected cells were compared to untransfected HEK293T cells. Five replicates were performed for each condition.