CLiP Belize Microplastics in biota 2019

This dataset contains 2 csv files. One file contains data on microplastic abundance expressed in number of particles from biota samples (queen conch, Lobatus gigas) collected in Belize in 2018 (and analysed in 2019) with indication of sample wet weight and blank used. The other file contains the profiles of the ATR-FT-IR spectrum analysis of plastic pieces found in the conch stomachs. A README text file contains the legend of the columns of the two csv files. A .zip file contains pictures of two plastic pieces analysed with ATR-FT-IR. The Commonwealth Litter Project (CLiP) supported Belize to take action on plastics entering the oceans. The assessment of microplastics in biota was part of the action plan to define scientific baselines for future monitoring purposes and comparison. Samples were acquired targeting the queen conch Lobatus gigas from Fishing Areas 1 and 2 (fished in Dec 2018), as defined by the Fisheries Department. Specimens were sourced as part of the Commonwealth Marine Economies (CME) programme. In the lab, stomachs were removed, rinsed and chemically digested by a 30 percent KOH:NaClO solution with an incubation period of 3 days 24 hours at 40 degrees centigrade. All samples were then filtered, stained with Red Nile dye and a digital image was acquired through a microscope. Number of particles was counted and corrected for particles count in blank samples. Visible particles were analysed using ATR-FT-IR to identify polymer composition, comparing their spectrum to a polymers library. ATR-FT-IR is the attenuated total reflection Fourier Transform infrared spectroscopy. A Thermo Fisher Scientific Nicolet iS5 ATR-FTIR with an OMNIC software (version 9.9.473) was used and polymers were identified based on the percentage match of IR spectra to a polymer library. Only spectra matched greater than 70 percent were accepted. Spectra were collected in the range 4000 – 650 1/cm at a resolution of 4 1/cm.

本数据集包含2个CSV文件。其中一份文件记录了2018年在伯利兹采集、2019年开展分析的生物样本（女王凤凰螺，学名*Lobatus gigas*）中的微塑料丰度数据，单位为颗粒数，同时标注了样本湿重及所用空白对照。另一份文件则收录了在该海螺胃内检出的塑料碎片的衰减全反射傅里叶变换红外光谱（Attenuated Total Reflection Fourier Transform Infrared Spectroscopy, ATR-FT-IR）分析谱图信息。另有一份README文本文件，用于说明两份CSV文件的各列数据含义。另有一个ZIP压缩包，包含了经ATR-FT-IR分析的2块塑料碎片的实拍图片。英联邦海洋废弃物项目（Commonwealth Litter Project, CLiP）为伯利兹应对海洋塑料污染提供了支持。本次生物体内微塑料评估，是为未来监测与对比工作确立科学基线的行动计划的组成部分。样本采集自渔业部门划定的1号、2号捕捞区域，目标物种为女王凤凰螺*Lobatus gigas*，捕捞工作于2018年12月完成。本次采样隶属于英联邦海洋经济项目（Commonwealth Marine Economies, CME）。实验室阶段，研究人员取出海螺胃组织，经漂洗后使用30%氢氧化钾-次氯酸钠溶液进行化学消解，消解过程于40℃下持续3天24小时。随后对所有样本进行过滤，并用尼罗红染料染色，通过显微镜采集数字图像。统计颗粒数量，并以空白样本中的颗粒数作为基准进行校正。对可见颗粒采用ATR-FT-IR进行聚合物成分鉴定，将其光谱与聚合物谱库进行比对。实验使用赛默飞世尔科技（Thermo Fisher Scientific）Nicolet iS5型ATR-FTIR光谱仪，搭配OMNIC软件（版本号9.9.473）完成检测；聚合物鉴定依据红外光谱与谱库的匹配度完成，仅匹配度高于70%的结果被采纳。光谱采集范围为4000~650 cm⁻¹，分辨率为4 cm⁻¹。