Supporting data for Cellular and molecular dysregulations of neutrophils in systemic lupus erythematosus.zip

(1) Clinical research data: Database containing patients’ data including patients’ clinical parameters are organized by our clinical research assistants with restricted access. Patients’ clinical data are anonymised and used for correlation analysis with laboratory data by GraphPad prism. (2) Laboratory research data 1) Flow cytometry: The data are automatically saved in .LMD or .fcs files. Then, the raw data will be copied by thumb drives and analysed by FlowJo software. After analysis, data can be saved in .wsp files. 2) qPCR: The raw data are saved in the .eds format and can be exported as .xls files. Excel and GraphPad prism are used for further analysis. Data collection of qPCR will follow the minimum information for publication of quantitative real-time PCR experiments (MIQE) guidelines. 3) Western Blot: PVDF membranes are scanned by ChemiDoc Imaging System (Bio-Rad). The raw data are saved in .scn files, the images are saved in .jpg and .tif formats. Further quantification is performed by Bio-Rad New Image Lab Software. 4) Immunofluorescence staining: Images are taken by Nikon Ni-U fluorescence microscope and saved in .tif files. 5) ELISA, Bradford and Endotoxin detection assay: Microplate reader is used to detect and process biological and chemical data using absorbance. Data are exported in .xls files. 6)Pyrosequencing: Data are processed and exported in .xls files. 7) ATAC-sequencing assay: Data are processed and exported in .xls and .gz files.

(1) 临床研究数据集：本数据集由临床研究助理整理编制，涵盖患者临床参数等相关信息，并设置了访问权限管控。患者临床数据均已完成匿名化处理，将依托GraphPad Prism开展与实验室检测数据的相关性分析。
(2) 实验室研究数据集：
1) 流式细胞术（Flow Cytometry）：数据将自动保存为.LMD或.fcs格式文件，原始数据将通过U盘拷贝，并使用FlowJo软件开展分析，分析完成后可保存为.wsp格式文件。
2) 定量实时PCR（quantitative real-time PCR，qPCR）：原始数据以.eds格式存储，支持导出为.xls格式文件，后续将借助Excel与GraphPad Prism完成分析。qPCR的数据采集严格遵循《定量实时PCR实验发表最低信息规范（MIQE）》。
3) 蛋白质印迹（Western Blot）：聚偏氟乙烯（PVDF）膜经Bio-Rad ChemiDoc成像系统扫描，原始数据存储为.scn格式文件，成像结果保存为.jpg及.tif格式，后续将借助Bio-Rad New Image Lab软件完成定量分析。
4) 免疫荧光染色：图像由尼康Ni-U荧光显微镜采集，保存为.tif格式文件。
5) 酶联免疫吸附实验（ELISA）、Bradford蛋白定量检测与内毒素检测：采用酶标仪通过吸光度法完成生物与化学数据的检测与处理，检测数据将导出为.xls格式文件。
6) 焦磷酸测序（Pyrosequencing）：测序数据经处理后导出为.xls格式文件。
7) 转座酶可及性染色质测序实验（ATAC-sequencing assay）：测序数据经处理后导出为.xls及.gz格式文件。