Molecular identification of human and plant pathogens in municipal domestic wastewater for hydroponic system applications

Water is essential for human survival and socio-economic development, yet its overconsumption threatens global food security and ecosystem integrity. This necessitates a 60% increase in food production, further straining water resources. Hydroponic systems represent a promising solution, utilizing up to 90% less water than traditional methods while providing optimal growing conditions for crops. This study aimed to develop a PCR-based detection system for main human and plant pathogens in hydroponic systems using treated domestic wastewater. Metagenomic analysis of wastewater samples revealed significant microbial diversity, identifying human pathogens such as Pseudomonas aeruginosa and Yersinia enterocolitica, alongside plant pathogens including Rhodococcus fascians.