Additional file 2 of Genome-resolved evidence for functionally redundant communities and novel nitrogen fixers in the deyin-1 hydrothermal field, Mid-Atlantic Ridge

Additional file 1:. Table S1 Information regarding the samples, metagenomes, and assemblies in the current study. Table S2 219 high-quality MAGs (a) and 615 MAGs (b) obtained in the current study. Table S3 Information regarding the functional gene abundance in the samples TVG10 (a), TVG11 (b), TVG12 (c), and TVG13 (d). The abundance of each gene harbored by each taxon is normalized by RPKM (mapped reads per kilobase scaffolds per million sequenced reads) method. Table S4 Information regarding the abundances of CAZymes and secreted CAZymes in the samples TVG10 (a), TVG11 (b), TVG12 (c), and TVG13 (d). The abundance of each gene harbored by each taxon is normalized by RPKM (mapped reads per kilobase scaffolds per million sequenced reads) method. Table S5 Taxonomic details of this study and reference active and inactive samples that were used in the Figure 2 and S7b. Table S6 The accession ID and taxonomic information of the NifH sequences used in Figure 5a. Supplementary Data 1 Phylogenetic tree of bacterial MAGs based on 120 bacterial marker genes in newick format. The values depicted on the branches are bootstrap values. Supplementary Data 2 Phylogenetic tree of archaeal MAGs based on 122 archaeal marker genes in newick format. The values depicted on the branches are bootstrap values. Supplementary Data 3 Phylogenetic tree of 16S rRNA genes in newick format. The values depicted on the branches are bootstrap values. Supplementary Data 4 Phylogenetic tree of MAGs based on 16 ribosomal protein sequences in newick format. The values depicted on the branches are bootstrap values.

附加文件1：表S1 本研究中涉及的样本、宏基因组及组装序列相关信息。表S2 本研究获得的219条高质量宏基因组组装基因组（MAGs，Metagenome-Assembled Genomes）(a)与615条MAGs(b)。表S3 样本TVG10(a)、TVG11(b)、TVG12(c)及TVG13(d)中的功能基因丰度相关信息。各分类单元携带的每个基因的丰度通过RPKM（每百万测序reads对应的每千碱基支架的比对reads数，mapped reads per kilobase scaffolds per million sequenced reads）方法进行标准化。表S4 样本TVG10(a)、TVG11(b)、TVG12(c)及TVG13(d)中的碳水化合物活性酶（CAZymes，Carbohydrate-Active enZymes）及分泌型CAZymes的丰度相关信息。各分类单元携带的每个基因的丰度通过RPKM方法进行标准化。表S5 本研究及用于图2和图S7b的参考活性、非活性样本的分类学详情。表S6 图5a中使用的固氮酶铁蛋白（NifH，Nitrogenase Iron Protein）序列的登录号及分类学信息。补充数据1 基于120个细菌标记基因构建的细菌MAGs系统发育树，格式为newick，分支上的数值为自举支持值。补充数据2 基于122个古菌标记基因构建的古菌MAGs系统发育树，格式为newick，分支上的数值为自举支持值。补充数据3 基于16S rRNA基因的系统发育树，格式为newick，分支上的数值为自举支持值。补充数据4 基于16个核糖体蛋白序列构建的MAGs系统发育树，格式为newick，分支上的数值为自举支持值。