Effect of dietary macronutrients and immune challenge on gut microbiota, physiology, and feeding behavior in zebra finches

To increase our understanding of how dietary macronutrients affect physiology and gut microbiota and investigate whether feeding behavior is influenced by an immune threat, we conducted two experiments. In the first experiment, we determined if zebra finches (Taeniopygia guttata) exhibit shifts in physiology and gut microbiota when fed diets differing in macronutrient ratios. In our second experiment, we simulated an infection in birds using the bacterial endotoxin lipopolysaccharide (LPS) and quantified feeding behavior in immune challenged and control individuals, as well as birds housed near either a control pair (no immune threat), or birds housed near a pair given an immune challenge with LPS (social cue of heightened infection risk). We also examined whether social cues of infection alter physiological responses relevant to responding to an immune threat, an effect that could be mediated through shifts in feeding behavior. This dataset describes the meta data for the manuscript. The sequences for the manuscript have been uploaded to GenBank (BioProject accession number: PRJNA1112869).

为加深对膳食宏量营养素如何影响生理与肠道菌群的认知，并探究摄食行为是否会受到免疫威胁的调控，我们开展了两项实验。第一项实验旨在探究斑胸草雀（Taeniopygia guttata）在饲喂宏量营养素比例各异的日粮时，其生理状态与肠道菌群是否会发生改变。第二项实验通过细菌脂多糖（lipopolysaccharide, LPS）模拟鸟类感染状态，对免疫挑战组与对照组个体的摄食行为进行量化分析；同时分别检测了饲养于无免疫威胁的对照配对旁、以及饲养于接受LPS免疫挑战配对旁（即暴露于感染风险升高的社会信号）的鸟类的摄食行为。此外，我们还检验了感染的社会信号是否会改变与免疫应答相关的生理反应，该效应可通过摄食行为的改变介导实现。本数据集对应该研究稿件的元数据，相关序列已上传至GenBank（生物项目收录号：PRJNA1112869）。