five

sortChIC testdata package

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DataCite Commons2025-12-17 更新2024-08-18 收录
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https://figshare.com/articles/dataset/sortChIC_testdata_package/23544774/1
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<br> Test data from the protocol single-cell sortChIC, presented in Zeller, Yueng et. al., Nat. Gen . This dataset includes BAM files that represent MNase cuts targetted at **H3K27me1** histone mark in single-cells from adult mouse bone marrow, sorted into 384 well plates, along with associated metadata.  <br> List of contents:  - <strong>4x BAM files</strong> (indexed), that contain data from 4x 384-well plates, reads are subsetted for **chromosome 1** (mm10/GRCm38)  - <strong>mm10_chr1.2bit:</strong> 2-bit file storing the sequence information for mouse chromosome 1  - <strong>mm10_blacklist.bed:</strong> blacklisted regions to avoid  - <strong>sortChIC_barcodes.txt:</strong> barcodes corresponding to the 384-well plate  - <strong>metadata.txt:</strong> metadata file that defines cell types from FACS label <br> Links for reference:   - https://www.nature.com/articles/s41588-022-01260-3  - https://github.com/vivekbhr/sincei

本数据集源自Zeller、Yueng等学者发表于《自然·遗传学》(*Nat. Gen.*)的单细胞sortChIC实验方案的测试数据。该数据集包含成年小鼠骨髓单细胞中靶向**H3K27me1**组蛋白修饰的微球菌核酸酶(MNase)酶切位点数据的BAM(Binary Alignment Map)文件,这些细胞已被分选至384孔板中,同时附带相关元数据。 数据集内容清单如下: - 4个索引化BAM文件:涵盖4块384孔板的测序数据,仅保留了小鼠染色体1(mm10/GRCm38)的测序读段; - mm10_chr1.2bit:存储小鼠染色体1序列信息的2-bit格式文件; - mm10_blacklist.bed:需规避的基因组黑名单区域文件; - sortChIC_barcodes.txt:对应384孔板的条形码信息文件; - metadata.txt:基于荧光激活细胞分选(FACS)标记定义细胞类型的元数据文件。 参考链接: - https://www.nature.com/articles/s41588-022-01260-3 - https://github.com/vivekbhr/sincei
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figshare
创建时间:
2023-06-20
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