Additional file 2: Figure S1. of P2X7 receptor mediates NLRP3-dependent IL-1β secretion and parasite proliferation in Toxoplasma gondii-infected human small intestinal epithelial cells

FHs 74 Int cells infected with T. gondii at higher infectious doses (MOI 20) reduced cell viability. FHs 74 Int cells were infected with the T. gondii GFP-RH strain at the indicated MOIs for 8 h, and the viability of FHs 74 Int cells were evaluated by an MTT assay. Figure S2. Toxoplasma gondii-induced IL-1β production in FHs 74 Int cells. FHs 74 Int cells were infected with T. gondii at an MOI of 10 for different time periods, and pro-IL-1β protein levels in the cell extracts were determined by Western blotting. a Representative blots presenting the protein levels of pro-IL-1β in mock-infected or T. gondii-infected FHs 74 Int cells (α-Tubulin served as an internal control for protein loading). b Bar plot depicting the pro-IL-1β/α-Tubulin ratio as determined by densitometric analysis of Western blotting and expressed as fold change compared with the mock-infection control. For all panels, data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the uninfected control. All data are representative of three independent experiments. (ZIP 1710 kb)

当FHs 74 Int细胞以较高感染复数（MOI 20）感染刚地弓形虫（Toxoplasma gondii）时，细胞活力出现降低。将FHs 74 Int细胞以指定感染复数感染刚地弓形虫GFP-RH株8小时，随后采用MTT法检测FHs 74 Int细胞的活力。图S2：刚地弓形虫诱导FHs 74 Int细胞产生IL-1β。将FHs 74 Int细胞以MOI 10感染刚地弓形虫并培养不同时长，通过蛋白质印迹法（Western blotting）检测细胞裂解液中IL-1β前体（pro-IL-1β）的蛋白水平。a：代表性印迹图，展示模拟感染组与刚地弓形虫感染组FHs 74 Int细胞中IL-1β前体的蛋白水平（α-微管蛋白α-Tubulin作为蛋白上样内参）。b：柱状图展示IL-1β前体与α-微管蛋白的比值，该比值通过对蛋白质印迹结果进行光密度分析得到，以模拟感染对照组为基准，以倍数变化表示。所有面板中的数据均以平均值±标准差（mean ± SD）呈现。与未感染对照组相比，*P < 0.05，**P < 0.01，***P < 0.001。所有实验结果均来自三次独立重复实验。（压缩包大小1710 kb）