Single-cell RNA sequencing of breast cancer patient-derived organoids

Patient-derived organoids have shown great promise as models of human tumors. However, a detailed single-cell description of the heterogeneity of these models is still lacking and limits their future clinical use. Here we performed a 10X single-cell RNA sequencing experiment to study the single-cell phenotype of breast cancer patient-derived organoids from 6 donors. Breast cancer organoids were grown for 1-2 weeks and were then harvested, dissociated into a single-cell suspension and sequenced.