Analysis of the antifungal potential of <em>Macrocybe titans</em> extract against <em>Candida albicans</em> - Supplementary material

Aim: To investigate the antifungal potential of <em>Macrocybe titans</em> extracts against <em>Candida albicans</em>. Material &amp; methods: Extracts were obtained as aqueous (EfraMat-22 and EfraMat-45) and methanolic/ethyl acetate fractions. Results: Broth microdilution and disk diffusion assays showed that EfraMat-45 provided the best results in terms of minimum inhibitory concentration. Scanning electron microscopy analysis revealed morphological changes and slight damage on the surfaces of cells exposed to EfraMat-45 at the MIC. Fluorescence microscopy analysis of the yeasts showed cell elongation. EfraMat-45 presented high levels of phenolic compounds and flavonoids, high antioxidant activity and absence of <em>in vitro</em> cytotoxicity. Conclusion: The results indicated that the aqueous extract of <em>M. titans</em> is highly promising as an antifungal agent.研究目的：探究巨大杯伞（Macrocybe titans）提取物对白色念珠菌（Candida albicans）的抗真菌活性。 材料与方法：提取物分为水提组分（EfraMat-22与EfraMat-45）以及甲醇/乙酸乙酯分级萃取组分。 研究结果：肉汤微量稀释法与纸片扩散法实验结果显示，EfraMat-45在最低抑菌浓度（Minimum Inhibitory Concentration, MIC）方面表现最优。扫描电子显微镜分析表明，经MIC浓度EfraMat-45处理的酵母菌细胞表面出现形态学改变与轻微损伤。荧光显微镜分析受试酵母菌后发现，细胞出现伸长现象。EfraMat-45中酚类与黄酮类化合物含量较高，抗氧化活性较强，且无体外（in vitro）细胞毒性。 研究结论：本研究结果证实，巨大杯伞的水提组分具备开发为抗真菌制剂的巨大潜力。