Additional file 1 of Genotyping by sequencing for estimating relative abundances of diatom taxa in mock communities

Additional file 1: Table S1. The number of reads and clusters processed during the cluster filtering step of the msGBS pipeline. Table S2. Light microscopy counts by the diatom expert based on length, width, and fibula density. Table S3. Summary of morphometric measurements in this study compared with earlier measurements. Table S4. Light microscopy cell counts of monoclonal samples for the preparation of mixed (mock and calibration) samples. Table S5. Calculated volumes (microliters) of monoclonal samples containing the required number of cells for the preparation of mixed (mock and calibration) samples. Table S6. Estimated number of cells in calibration and mock samples based on Neubauer counts. Table S7. Expected and observed relative abundance estimates for mock samples using GBS and LM methods.

附加文件1：表S1。msGBS分析流程的聚类过滤步骤中处理的读段(reads)与聚类簇(clusters)数量。表S2。硅藻领域专家基于细胞长度、宽度及肋突密度开展的光学显微镜(Light Microscopy, LM)计数结果。表S3。本研究形态计量学测量结果与既往相关研究测量结果的对比汇总。表S4。用于制备混合（模拟与校准）样本的单克隆样本的光学显微镜细胞计数数据。表S5。为制备混合（模拟与校准）样本，需配制含指定细胞数的单克隆样本的计算体积（单位：微升）。表S6。基于牛鲍计数板(Neubauer)计数估算的校准样本与模拟样本中的细胞总数。表S7。采用GBS与光学显微镜(LM)方法得到的模拟样本的预期与观测相对丰度估算值。