Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards

Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards In this community effort, we compared measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as new suite of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method (SOP) and/or their method of choice (OTHER). Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. The attached file "ILS-Ceramide-Ring-Trial-Datasets.csv" and the table below map the lab number (LabNum) used in the manuscript in all plots to the originally assigned submission Id (LabId) used in the anonymized reports containing the peak areas submitted by each lab for their SOP (Standard) and / or OTHER (Preferred) workflow. The table below provides further information on the separation used (LC), the mass analyzer type (QQQ=Triple Quads and Traps, Orbitrap, TOF) and the associated mass analyzer resolution (LowRes, HighRes), and links each LabNum to the corresponding dataset name and Zenodo DOI, if available. In order to retain the anonymity of all participating labs w.r.t. the submitted datasets, only the converted mzML files are provided in the linked submissions. Please note that labs were free to choose whether they wanted to disclose their MS data or not. Thus, missing datasets indicate that the corresponding lab did not provide their raw / mzML data. All reports together with the code for analysis and visualization, reproducing the figures in the manuscript, are available under the following doi: https://zenodo.org/doi/10.5281/zenodo.10081970. This links to releases of the following GitHub repository: https://github.com/lifs-tools/ils-ceramide-ring-trial. Ring Trial mzML Datasets LabNum LabId Protocol LC MassAnalyzerType MassAnalyzerResolution DatasetName DOI 1 02b SOP RP QQQ LowRes Lab_01_SOP 10.5281/zenodo.13134264 2 3 OTHER RP QQQ LowRes Lab_02_OTHER 10.5281/zenodo.13145059 3 4 SOP RP QQQ LowRes Lab_03_SOP 10.5281/zenodo.13134437 4 5 OTHER RP QQQ LowRes Lab_04_OTHER 5 7 OTHER RP Orbitrap HighRes Lab_05_OTHER 10.5281/zenodo.13134439 6 9 OTHER FIA QQQ LowRes Lab_06_OTHER 10.5281/zenodo.13134441 7 10a SOP RP QQQ LowRes Lab_07_SOP 10.5281/zenodo.13134445 7 10b OTHER RP QQQ LowRes Lab_07_OTHER 10.5281/zenodo.13134443 8 12 SOP RP QQQ LowRes Lab_08_SOP 10.5281/zenodo.13134447 9 13 OTHER RP QQQ LowRes Lab_09_OTHER 10 14 SOP RP QQQ LowRes Lab_10_SOP 11 15 SOP RP QQQ LowRes Lab_11_SOP 10.5281/zenodo.13134449 12 16 OTHER RP Orbitrap HighRes Lab_12_OTHER 10.5281/zenodo.13134451 13 17a OTHER RP TOF HighRes Lab_13_OTHER 14 18a SOP RP QQQ LowRes Lab_14_SOP 10.5281/zenodo.13135617 14 18b SOP RP QQQ LowRes Lab_14_SOP 10.5281/zenodo.13135617 15 19 SOP RP QQQ LowRes Lab_15_SOP 10.5281/zenodo.13135625 16 20a SOP RP QQQ LowRes Lab_16_SOP 16 20b OTHER RP QQQ LowRes Lab_16_OTHER 17 21 SOP RP QQQ LowRes Lab_17_SOP 18 22 SOP RP QQQ LowRes Lab_18_SOP 10.5281/zenodo.13135627 19 23 SOP RP QQQ LowRes Lab_19_SOP 10.5281/zenodo.13135629 20 24 SOP RP QQQ LowRes Lab_20_SOP 21 25 SOP RP QQQ LowRes Lab_21_SOP 10.5281/zenodo.13244007 22 26a OTHER FIA Orbitrap HighRes Lab_22_OTHER 22 26b OTHER FIA QQQ LowRes Lab_22_OTHER 23 27 SOP RP QQQ LowRes Lab_23_SOP 10.5281/zenodo.13135632 24 28 SOP RP QQQ LowRes Lab_24_SOP 10.5281/zenodo.13135634 25 29a SOP RP TOF HighRes Lab_25_SOP 10.5281/zenodo.13136167 25 29b OTHER SFC QQQ LowRes Lab_25_OTHER 10.5281/zenodo.13135638 26 30 SOP RP QQQ LowRes Lab_26_SOP 10.5281/zenodo.13136171 27 31 SOP RP QQQ LowRes Lab_27_SOP 10.5281/zenodo.13136176 28 32 OTHER RP TOF HighRes Lab_28_OTHER 29 33 OTHER RP QQQ LowRes Lab_29_OTHER 30 34 SOP RP QQQ LowRes Lab_30_SOP 31 35 OTHER RP QQQ LowRes Lab_31_OTHER 32 36 SOP RP QQQ LowRes Lab_32_SOP 10.5281/zenodo.13166454 33 37 OTHER RP QQQ LowRes Lab_33_OTHER 10.5281/zenodo.13732469 34 38 SOP RP QQQ LowRes Lab_34_SOP 10.5281/zenodo.13324807