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Additional file 5: Supplemental Tables. of Iron homeostasis in Arabidopsis thaliana: transcriptomic analyses reveal novel FIT-regulated genes, iron deficiency marker genes and functional gene networks

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Table S1. List of genes that were found regulated in the inter-line and intra-line comparisons in six-day-old seedlings and roots of six-week-old plants and that were found in iron homeostasis-related clusters after hierarchical clustering of the seedling, root and combined expression data. Table S2. Lists of input genes and the output genes before and after each of the four filtering steps. The lists are sorted alphabetically. Table S3. Expression patterns of genes that are induced by FIT in six-day-old seedlings. The genes marked red were found regulated in seedlings but not in the root samples. The genes marked yellow have previously been associated with iron homeostasis. For comparison, the respective root expression patterns have been added to the right. Additionally, we have added a column (S) with selected publications in which mutants have been investigated or which establish a direct link to iron homeostasis. Table S4. Expression patterns of genes that are induced by FIT in roots of six-week-old plants. The genes marked red were found regulated in the root samples but not in seedlings. For comparison the respective seedling expression patterns have been added to the right. Table S5. Expression patterns of genes that are repressed by FIT in six-week-old roots. The genes marked red were found regulated in the root samples but not in seedlings. For comparison the respective seedling expression patterns have been added to the right. Table S6. Expression patterns of genes that are repressed by FIT in six-day-old seedlings. The genes marked red were found regulated in seedlings but not in the root samples. For comparison the respective seedling expression patterns have been added to the right. Table S7. List of genes that were found in common regulons with ≥ 4 nodes in the co-expression analysis of iron deficiency-induced genes in the virtual dataset. The numbers correspond to the numbering in Fig. 7. Table S8. List of primers which we used to validate the expression data of some iron homeostasis-related genes. (XLSX 197 kb)

表S1 基因列表:在6日龄幼苗以及6周龄植株根系的品系间与品系内比较中呈现表达差异的基因,且经对幼苗、根系及整合表达数据进行层级聚类后,被归入铁稳态相关聚类簇中。 表S2 输入与输出基因列表:包含四次过滤步骤各自执行前后的输入基因与输出基因列表,所有列表均按字母顺序排序。 表S3 6日龄幼苗中受FIT(FIT)诱导的基因的表达模式:标注为红色的基因仅在幼苗样本中呈现表达差异,未在根系样本中检测到;标注为黄色的基因此前已被证实与铁稳态相关。为便于对比,右侧补充了对应根系样本的表达模式数据。此外,本表格新增(S)列,收录了相关研究文献——这些文献要么对相关突变体开展了功能验证,要么直接建立了与铁稳态的关联。 表S4 6周龄植株根系中受FIT(FIT)诱导的基因的表达模式:标注为红色的基因仅在根系样本中呈现表达差异,未在幼苗样本中检测到。为便于对比,右侧补充了对应幼苗样本的表达模式数据。 表S5 6周龄植株根系中受FIT(FIT)抑制的基因的表达模式:标注为红色的基因仅在根系样本中呈现表达差异,未在幼苗样本中检测到。为便于对比,右侧补充了对应幼苗样本的表达模式数据。 表S6 6日龄幼苗中受FIT(FIT)抑制的基因的表达模式:标注为红色的基因仅在幼苗样本中呈现表达差异,未在根系样本中检测到。为便于对比,右侧补充了对应幼苗样本的表达模式数据。 表S7 虚拟数据集中铁缺陷诱导基因共表达分析中,节点数≥4的共调控子(regulon)共有基因列表。编号与图7中的编号一一对应。 表S8 用于验证部分铁稳态相关基因表达数据的引物列表。 (XLSX格式,文件大小197 KB)
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Figshare
创建时间:
2016-12-15
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