The sequences of all forward and reverse PCR primers used in this study are highlighted along with their corresponding melting temperatures. The junction spanning details of the VEGFxxxb isoform-speci
*p-value for mean total RNA concentration between methods. Total RNA concentration and purity for Trizol LS and Qiagen miRNeasy with RNA yeast carrier.
aall forward primers (top) included the following common sequence at the 5′ end: AGGAAGAGAG; all reverse primers (bottom) the sequence CAGTAATACGACTCACTATAGGGAGAAGGCT.
