RNA-seq data.xlsx

Maternal obesity was induced by feeding high-fat diet feed for 10 weeks in female C57/BL6 mice, while control mice were fed a standard diet. All female mice mated with healthy male mice and allowed to deliver spontaneously. A subset of female offspring (3 from control dams and 3 from obese dams) were sacrificed by cervical dislocation at 3 weeks of age, and liver samples were used for RNA-sequencing (mRNA and lncRNA) analysis.

本研究以雌性C57/BL6小鼠为实验对象，通过饲喂高脂饲料10周构建母体肥胖模型，对照组小鼠则饲喂标准饲料。所有雌性小鼠均与健康雄性小鼠合笼交配，并允许其自然分娩。选取部分雌性子代小鼠（对照组母鼠所产后代3只、肥胖母鼠所产后代3只）于3周龄时采用颈椎脱臼法处死，采集肝脏样本用于RNA测序（信使RNA（mRNA）与长链非编码RNA（lncRNA））分析。