Assessment of dCAS9 gene expression in the strains under study.
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qPCR data are represented as cycle numbers. Higher number represents lower expression. All “no template controls” and “no RT controls” showed no amplification and were omitted from the table. WT: Wild type; X: plasmid with no spacer. The spacers in the plasmids are as follows. O and OO: single and double spacers against psbO; A: spacer against psbA.
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2019-11-26



