Results for the bioinformatics analysis on the matching of SB primers to NUMTs.

The 17 SB amplicons were designated as A to P amplicons by Bannwarth et al., but we have reassigned it to SB#1 to SB#17 for better illustration. Amp. # represents amplicon #. Primer sequences were obtained from the paper of Bannwarth et al. [11]. F and R represent forward primer and reverse primer of each amplicon, respectively. Hits on NCBI and Hits on BioEdit indicate the number of hits with perfect match from the primer sequences with nDNA sequences in the GenBank database of the NCBI and the local database created in BioEdit, respectively, according to the BLAST search results. The possibility of 2 primers for each amplicon matching with nDNA on the same chromosome was evaluated, and the matched accession numbers, positions on the chromosome, and the sizes of the putative NUMTs are indicated. Match for NUMT location refers to the conditions of matching both primers to the same chromosome, with the following symbols indicating the matching conditions of the primers to nDNA: •, perfect match for both primers to the accession number indicated; △, perfect match for one primer and one mismatch for another primer; ▽, perfect match for one primer and 2 or more mismatches for another primer; ▾, one mismatch for one primer and 2 or more mismatches for another primer. N and L indicate results from NCBI and local database searches, respectively. The symbol of — indicates that no matching result for the same chromosome could be found. Although some primers could match the same chromosome, even with 2 or more mismatches, the matched primers could be positioned too far away from each other to practically generate PCR products. In such cases, the matching results are not listed.