RNA-seq of the copepod Pseudocalanus newmani using rRNA-depletion approach

RNA-seq data using rRNA-depletion approach were obtained for the copepod Pseudocalanus newmani to detect major viruses associated with copepods. Zooplankton samples were collected using NORPAC net with a 335-µm mesh size at the Okhotsk Tower, located 1 km off the coast of Mombetsu, northeastern Hokkaido, Japan. Adult female P. newmani were picked up and preserved in RNAlater after incubation to depurate the copepod guts. A total of 100 P. newmani individuals were used for RNA extraction using a Direct-Zol RNA MiniPrep Kit (Zymo Research). RNA quality was assessed using the 4200 TapeStation (Agilent Technologies), and RNA concentration was measured using a Qubit 3.0 Fluorometer (Life Technologies). After rRNA removal, a sequence library was prepared using the MGIEasy rRNA Depletion Kit, MGIEasy RNA Directional Library Prep Set, MGIEasy Circularization Kit, and High-throughput Sequencing Kit (MGI Tech Co., Ltd.). Sequence data of 2 � 150 bp paired-end were obtained using DNBSEQ-G400 (MGI Tech Co., Ltd.).