Transcriptome analysis of liver tissues was performed on the control group, alcohol-induced ALD models, and mice treated with kaempferol copper complex using RNA sequencing.

Alcohol consumption can induce liver damage and inflammation. However, the development of therapeutic strategies and drugs for alcoholic liver disease (ALD) is still in early stages. Kaempferol is considered a potential ALD treatment candidate due to its excellent antioxidant and anti-inflammatory activities. Preliminary studies show the kaempferol-copper complex (Ka-Cu) exhibits enhanced pharmacological activity compared to free kaempferol, though its precise molecular mechanisms remain unclear. In this study, transcriptomic sequencing of liver tissue revealed Ka-Cu intervention significantly inhibited TLR4 and MyD88 protein expression, thereby suppressing downstream MAPK and NF-kappa B signaling pathways. Our research elucidates a novel regulatory mechanism of Ka-Cu in alcohol-induced acute liver injury via the TLR4/MAPK/NF-kappa B axis. This study provides insights into Ka-Cu's hepatoprotective mechanisms and identifies potential therapeutic targets.