Transcriptome and DNA methylome of mouse Müller glia

Müller cells are the primary glia of the neural retina and play crucial roles in maintaining the structural and functional homeostasis of the retina. Müller cells also possess certain levels of retinal regeneration capacity, especially in lower vertebrates. In this study, we deep sequenced the transcriptomes and methylomes of mouse Müller cells and early retinal progenitor cells (RPCs), as well as Müller cells from injured retinas and aged retinas, which will help to reveal molecular mechanisms governing Müller cells development, physiological functions and regeneration activities. Overall design: Total RNAs were extracted and deep sequenced (2 biological replicates each for young Müller cells and aged Müller cells, 3 replicates each for NMDA-treated Müller cells and RPCs). Methylomes of genomic DNAs were deep sequenced using RRBS method (3 biological replicates each for young Müller cells, NMDA-treated Müller cells, aged Müller cells, and RPCs).