FGF19 induces the inflammatory phenotype of LX-2 cells

Liver metastasis is the main cause of death for human colorectal cancer (CRC), thus effective biomarkers and therapeutic target of colorectal cancer liver metastasis (CRLM) are urgently needed. Here, we report that FGF19 mediated the polarization of hepatic stellate cells to inflammatory CAFs by activating the autocrine effect of IL-1a via JAK2-STAT3 pathway. FGF19-induced iCAFs produced complement C5a and IL-1ß to enhance neutrophil extracellular traps (NETs) formation in liver metastases, which in turn accelerated the liver colonization of CRC cells. Overall design: LX-2 cells were stimulated with hFGF19(50 ng/ml or 100 ng/ml) for 24 hours and subjected to differential gene expression studies by RNA-seq.