five

whole genome sequencing of evolved CRISPRi-CAS12m strains

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1184198
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To assess the genetic stability of a CRISPRi-Cas12m biosecure design, we conducted a long-term growth experiment, passaging the strain daily under permissive conditions for 21 days. Whole-genome sequencing was performed on both the original and evolved strains to identify any mutations that may have occurred within the three Cas12m insertions in the E. coli chromosome.
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2024-11-09
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