Neuron Morphology data in tiff stack format from Patch-seq experiments in human neocortex, Cell ID 855488261

Human neocortical neurons display diverse morphology, physiology and gene expression profiles. We use a method called Patch-seq, where these three data modalities are assayed simultaneously from the same neuron, to understand how the genetic profile of human neocortical neuron types corresponds to their morphological and electrophysiological phenotypes. This collection may contain image stacks (TIFF), reconstruction files in image space (.swc, µm), a transformed file (.swc, µm) oriented so that the y axis extends vertically towards the pia surface, and a marker file (.marker, µm) indicating nodes where dendrites were truncated (10), reconstruction was not continued (20), or surface of tissue was closest to soma (30).

人类大脑新皮层神经元呈现出多样化的形态学、生理学与基因表达特征。我们采用名为膜片钳测序（Patch-seq）的技术，可从同一神经元中同时获取上述三类数据模态，以此探究人类新皮层神经元类型的基因特征与其形态学及电生理学表型之间的对应关系。本数据集集合可能包含以下类型文件：图像堆栈文件（TIFF格式）、图像空间重建文件（.swc格式，单位：微米）、经坐标转换的重建文件（.swc格式，单位：微米，其y轴垂直指向软脑膜表面），以及标记文件（.marker格式，单位：微米）。该标记文件用于标注三类节点：树突被截断的节点（标记值10）、重建过程未继续的节点（标记值20），以及组织表面最接近神经元胞体的节点（标记值30）。