The effect of dietary soybeans and their isoflavone content on the expression profiles of mRNAs in liver of rats

The GeneChip data was normalized and summarized by using SuperNORM data service (Skylight Biotech Inc.). Significance of expressional change among groups was tested by 2-way ANOVA on the normalized CEL data, which was deposited in a tab-separated ASCII text format. Principal components were identified on the summarized gene data.本研究使用Skylight Biotech Inc.提供的SuperNORM数据服务（SuperNORM data service），对基因芯片（GeneChip）数据进行标准化与汇总处理。针对以制表符分隔的ASCII文本格式存储的标准化CEL数据（CEL data），采用双因素方差分析（2-way ANOVA）检验各组间基因表达变化的显著性；随后在汇总后的基因数据上识别主成分（Principal components）。