H3K36 methyltransferase SETD2 regulates lymphoid and neural development [seq]

We study the role of loss of Setd2 and H3K36me3 in early B cell development through NGS methods including RNA, ChIP and ATAC Seq of sorted B cell populations of control and Setd2KO primary B cells from mice. From age-matched littermate controls and Setd2KO mouse, we sorted early proB cells (B220+CD43+CD19-Cd25-IgM-) and performed ATAC-seq, RNA-Seq, and ChIP-seq using an antibody against H3K36me3.本研究采用下一代测序（Next Generation Sequencing, NGS）技术，对小鼠来源的对照组与Setd2基因敲除（Setd2KO）原代B细胞的分选群体开展RNA测序（RNA-seq）、染色质免疫共沉淀测序（ChIP-seq）与转座酶可及性测序（ATAC-seq），以此探究Setd2缺失与H3K36me3丢失在早期B细胞发育中的调控功能。本研究选取同窝同龄的对照组小鼠与Setd2KO小鼠，分选得到早期前B细胞（B220+CD43+CD19-CD25-IgM-），并使用针对H3K36me3的抗体完成ATAC-seq、RNA-seq与ChIP-seq实验。