Escherichia coli str. K-12 substr. MG1655 Transcriptome or Gene expression. Escherichia coli str. K-12 substr. MG1655

The goal was to establish a robust and scalable RNA-seq process applicable to cultured bacteria as well as to complex community transcriptomes. To this end, we evaluated rRNA depletion methods and chose a protocol that eliminates rRNA reads efficiently and robustly, and largely irrespective of the quality of the RNA input sample.